Good mothers provide comfort and protection to their offspring. We hypothesized that ewes with twin lambs would show changes in attention and grooming behaviours according to their offspring's stress and pain experience. The experiment was carried out under UK Home Office License. On day two after birth (D2) maternal behaviours (e.g. sniffing, licking and glancing) were recorded after 10 minutes of handling, in 31 pairs of lambs. This was used as a baseline comparison to behavioural data collected following adverse treatments on D3 and D4. For each day of treatment, we analysed pairs where one sibling was treated and the other remained as a control (handled only). Twenty-four pairs were used on D3 and 21 pairs were used on D4. On D3, the treated twin was stressed by either LPS injection (17/24) or 10 minutes of social isolation (7/24). On D4, the treated twin experienced pain. Nine out of 21 treated lambs and 7/21 controls on D4 had experienced stress on D3. The remaining lambs had experienced just handling on D3. Pain was induced by tail docking and castration in males (16/21) and tail-docking in females (5/21). LPS and social isolation data were pooled together for D3 analysis, as were tail-docking and castration data for D4. Using Wilcoxon rank sum test in JMP® (version 7.0.1, SAS Institute), we observed a higher frequency of "sniffing" (p=0.0032) and "glancing" (p=0.0001) by ewes towards treated lambs on pain day, compared to matched baseline measures recorded on the same animal, while no effect was found for other behaviours during either stress or pain treatments. In conclusion, ewes showed increased attention towards their offspring experiencing pain. Further studies will determine the effect of increased ewe attention on the