This study examined the physiological and behavioural effects of transport on young calves in a 3x2x3 factorial design comparing age, transport or no transport, and time. Eight calves at each of 3, 5 and 10 days-of-age were transported for 10 hours in individual pens. An additional 8 calves at each age were individually penned without access to food or water for 10 hours. Testing occurred over a 3-week period with 2 ages compared in each testing week. Blood sampling from indwelling catheters occurred immediately prior to loading onto the trailer or into a control pen at 0.5, 2, 4, 6 and 10h after loading, and 0.5, 12 and 24h after unloading. Lying, standing, walking and running were observed for 2h post-transport. Analysis of variance found no age, treatment, time or interaction effects. For example, time spent lying was not different between the transported (mean±sem; 108.95±4.62mins) and the control calves (101.69±4.39mins) (F(1,44)=1.296, p=0.263). Repeated measures analysis of variance showed significant age (F(2,46)=13.671, p=0.019) and time of testing (F(2,46)=4.875, p=0.027) main effects for total blood cortisol levels; 3-day-old calves had significantly higher levels (47.49±4.963nmol/L) than 10-day-old calves (18.47±4.723nmol/L) regardless of treatment. There were no significant treatment main effects, but there was a significant time x treatment interaction effect (F(8,28)=2.908, p=0.027). No main age, treatment or time of testing effects on white blood cell counts or packed cell volume were observed. Significant time (F(3,39)=3.660, p=0.020), time x treatment (F(3,39)=3.643, p=0.021) and treatment x age (F(2,41)=4.270, p= 0.021) interaction effects were found for lymphocyte counts, with lymphocyte counts increasing over time and across age although lymphocyte counts were within normal ranges at all times throughout the study. Therefore, the results suggest minimal impacts of individual transport under 'ideal' conditions on calves aged 3, 5 and 10 days.